pe arginase 1 arg1 antibody  (R&D Systems)

Journal: Journal of Biomedical Science

Article Title: Aptamer-based inhibition of MNK1 reduces pancreatic ductal adenocarcinoma growth by targeting cancer stem cells

doi: 10.1186/s12929-026-01275-6

Figure Lengend Snippet: apMNKQ2 targets the CSC compartment in PDAC. A, B Left: Representative images of colonies ( A ) or spheres ( B ) in control- or apMNKQ2-transfected Panc354 cells. Right: Mean ± SEM in the crystal violet optical density (OD) ( A ) or number (no.) of spheres/ml ( B ) in control- or apMNKQ2-transfected Panc215 or Panc354 cells. * p < 0.05, **** p < 0.0001; as determined by one-sample t test. C Mean ± SEM of the percentage of CD24-, CD133-, CXCR4- or ALDH-positive cells in control- or apMNKQ2-transfected cells 24 h post transfection. *** p < 0.001, **** p < 0.0001; as determined by one-sample t test. D Schematic of the transfection groups, apMNKQ2-FITC transfection efficiency determined by flow cytometry and subsequent dilution of cells for ELDA determination. E Percentage of tumor take (number of tumors confirmed/number of injections) for subcutaneously injected control (CTL)- and apMNKQ2 (Q2)-transfected cells was determined over the course of 124 days. F Tumor weight (g) o tumors extracted at indicated times in ( E ). ns = not significant; nd = not determined. G CSC frequencies determined using the extreme limiting dilution analysis algorithm ( http://bioinf.wehi.edu.au/software/elda/index.html ) (left, 95% CI) and images of resected tumors for the dilution 5 × 10 5 (right)

Article Snippet: For the detection of CD24, CD133 or CXCR4 cell surface marker expression, cells were incubated with a 1:5 dilution of a mouse anti-human CD24 PE (BD Cat no. 555428), a 1:50 dilution of a mouse anti-human CD133/1 Vio Bright R667 Ab (Miltenyi Cat no. 130–111–756) or a 1:50 dilution of a mouse anti-human CXCR4 (CD184) PE Ab (Miltenyi Cat no. 130–117–354).

Techniques: Control, Transfection, Flow Cytometry, Injection, Software